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Paper Details

Optimization of Chitinase Production Using Statistics Based Experimental Designs

Sudhakar.P* and Nagarajan.P

Journal Title:Journal of Chemical, Biological and physical sciences

Statistics based experimental design on chitinase production by Serratia marcescens was optimized in solid state fermentation using Plackett-Burman design and Response surface methodology. The important medium components identified by initial screening method of Plackett-Burman were colloidal chitin, yeast extract, MgSO4.7H2O and KH2PO4. Plackett-Burman Pareto chart illustrates the order of significance of the variables affecting the cellmass production. Central composite response surface methodology was performed to evaluate the effects of temperature, pH, inoculum size and substrate concentration on production of chitinase by Serratia marcescens was studied using sugarcane bagasse under solid state fermentation. Statistical analysis of results showed that, the linear and quadric terms of these four variables had significant effects and evident interactions existing between pH and substrate concentration were found to contribute to the response at a significant level. Under the conditions of pH (6), Temperature (30oC), inoculum size (2.4%) and substrate concentration (2 g) in the experiment. The predicted response for chitinase production was 52.72 U/ml