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Paper Details

Biodegradation of perchlorate by methylophaga sp. lmn developed from an acclimatized mixed microbial consortium

J.R. Anoop Raj1* and L. Muruganandam2

Journal Title:Journal of Chemical, Biological and physical sciences

Present study deal with the microbial degradation of perchlorate (ClO4 -) present in waste water using a pure culture developed from an acclimatized mixed microbial consortium. The microbial screening study shows that the strain LMN isolated from the mixed consortium has the capacity to degrade perchlorate. The effect of varying perchlorate concentration on strain LMN was studied using batch reactor under anaerobic environment. Growth kinetic study conducted over different perchlorate concentration range from 0 to 100 mg/L. The dry weight of cells and colony forming units per millilitre (CFU/ml) studies were carried out to determine the growth of bacteria. The dynamic growth rate of the strain LMN was identified in the logarithmic phase and a linear relationship between growth rate and perchlorate concentration calculated. A kinetic model was developed incorporating the cell density, growth rate and perchlorate degradation rate. Batch reactor studies shows that around 89.2% of perchlorate reduction was achieved within 9 h of incubation at an initial perchlorate concentration of 25 mg/L. Growth retardation effect was observed on strain LMN and found to be predominant at >50 mg/L. The relationship obtained between growth rate and perchlorate concentration proves that growth and degradation rate of bacteria were inversely proportional to the perchlorate level. The perchlorate concentration and biomass level are the important factors in the biodegradation mechanism. Study results prove that perchlorate as an environmental pollutant exhibit mild growth inhibitory effect towards Methylophaga sp. LMN and the above findings are useful for the development of perchlorate remediation systems.