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Paper Details

Quantitative Analysis of Quercetin in Pueraria Tuberosa by Using High Performance Liquid Chromatography

Bharti Jain* and Rashmi Raghuvanshi

Journal Title:Journal of Chemical, Biological and physical sciences

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method has been developed and described for the quantitative determination of quercetin the root tuber powder of Pueraria tuberosa. The analysis was done at room temperature with a Promosil C18 column (250mm4.60mm i.d.,5?m particle size) as stationery phase at a wavelength of 267nm for detection and determination. The mobile phase consisted of water: acetonitrile : acetic acid (90:10:0.2 v/v/v) at a flow rate of 1ml/min.The quercetin content of 66.7% was found in the ethanolic extract of crude Pueraria tuberosa. The proposed method is precise, sensitive, reproducible and easy to perform which can be effectively used for detection, monitoring and quantification of the flavonoid quercetin in Pueraria tuberosa.