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Paper Details

Studies on L-Asparaginase Production by Using Staphylococcus Capitis

Udaya Paglla1, C.S.V.R.Rao and Satish Babu Rajulapati2*

Journal Title:Journal of Chemical, Biological and physical sciences

L-Asparaginase is a therapeutic enzyme which is used in treatment of different types of cancers. Many bacteria are able to produce this enzyme but it has some side effects. If this enzyme is produced from microbe which is isolated from medicinal plants, side effects can be reduced. This paper presents the study on L-Asparaginase production by endophytic bacteria from Mentha Spicata. Four microbes were isolated and tested for enzyme production. One of these microbes shown positive results for L-Asparaginase production and it was identified as Staphylococcus capitis. Medium composition was optimized for the improvement of L-asparaginase activity by RSM (Response Surface Methodology). Effect of five process variables such as time, temperature, pH, inoculum size and substrate concentration were studied on enzyme activity. The optimized process variables were found to be pH 6.2, temperature 25C, substrate concentration 0.5 g, time 6 days and inoculum size was 3 ml. It was observed that L-asparaginase activity was improved from 83 to 224 U/ml after optimization.