In vitro studies on anti-cancer activity of anti-cancerous compound producing marine bacteria against on cancer cells by MTT assay
V.S.L.Mithun * and C.S.V.Rama Chandra Rao
Journal Title:Journal of Chemical, Biological and physical sciences
Extracts from microorganisms have served as a valuable source of diverse
molecules in many drug discoveries. Identification of microbial strains having
promising biological activities and purifying the bio-molecules which are responsible
for the biological activities, have led to the discovery of many bioactive molecules.
Extracts of bacteria in vitro tested on various cancer cell lines. The lyophilized
bacterial extract powder was dissolved in various chemical solvents like Methanol,
Chloroform and Ethyl acetate. From them cytotoxic assays was performed with the
extracts were screened on HCT 15 and MES- SA cancer cell lines to study the
cytotoxic potential. Where the Ethyl acetate showed the viability when compared to
other solvents. The Ethyl acetate extract of isolate showed promising results by MTT
assay and Trypan blue staining.