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Paper Details

Molecular and Biochemical Characterization of Ischemic Stroke

P.Vijay Amruth Raj and C.S.V.Rama Chandra Rao

Journal Title:Journal of Chemical, Biological and physical sciences

Stroke or cerebral infarction is a condition that arises due to the obstruction in the flow of blood (ischemic stroke) or due to rupture in the blood vessels supplying blood to the brain (hemorrhagic stroke). This results in lack of oxygen and nutrients to the brain cells causing their failure to perform the metabolic function. Stroke is the third largest killer in the World. The total damage arising from stroke depends on the area of the brain affected and the amount of blood obstructed to the brain. In the present study we aimed to study the single nucleotide polymorphism in phosphodiesterase 4D (PDE4D) SNP56 genes and LPL gene. 25 patients and 25 healthy individuals blood samples were collected and DNA was isolated. The presence of PDE4D SNP56 gene was determined by PCR-Sequencing methods and LPL gene polymorphism was determined by PCR-RFLP methods. Same numbers of patient and control samples were used to estimate the nitric oxide levels and uric acid levels. The results indicate that the PDE4D SNP56 gene and LPL gene polymorphism is significantly associated with stroke in the study population. The serum nitric oxide and uric acid levels were found to be significantly increased in patients as compared to controls.