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Paper Details

Isolation, Screening and Molecular Characterization of Chromium Reducing Cr (VI) Pseudomonas Species

R.Jayalakshmi*and C.S.V. Rama Chandra Rao

Journal Title:Journal of Chemical, Biological and physical sciences

Microorganisms are known for their ability to metabolize a variety of chemical compounds, including aliphatic and aromatic hydrocarbons, fatty acids and other environmental pollutants. These capabilities make them useful for application as bioremediation agents. Heavy metals found in wastewaters are harmful to the environment and their effects on biological system are very severe. An efficient and economic treatment for their removal and reuse needs to be developed. Microbial metal bioremediation is an efficient strategy due to its low cost, high efficiency and ecofriendly nature. Recently advances have been made in understanding metal-microbe interaction and their application for metal detoxification. Microorganisms in soil are sensitive to the high concentrations of heavy metals like zinc, manganese, cobalt, copper, chromium, cadmium, mercury and silver. Chromium is a toxic heavy metal, which primarily exists in two inorganic forms, Cr (VI) and Cr (III). Chromate [Cr (VI)] is a serious environmental pollutant. The current study aimed to isolate the bacteria which have the potential capacity to reduce the chromium levels. Chromium contaminated soil was collected and analyzed. From the collected soil samples the bacterial isolates were screened and characterized by using the 16s rRNA based PCR amplification. Biochemical tests were performed for the microorganisms isolated from the effluent. As per the present study the isolated Pseudomonas sp was found to reduce 99 % of chromium from the medium in 21 hours.