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Paper Details

Estimation of Cystiene Using Sodium Nitroprusside by a Newer Photochemical Method

1Shailendra Singh Rathore, Ravi Sharma2, Devendra Kadam3 and Ajay Sharma1*

Journal Title:Journal of Chemical, Biological and physical sciences

A newer fast, convenient and inexpensive quantitative method for the determination of Cystiene using photochemical exchange reaction of sodium nitroprusside has been investigated. Sodium nitroprusside is a photolabile complex and it undergoes photochemical ligand exchange reactions rapidly. Some recent efforts have been made to utilise such reactions for the estimation of some sulphur containing anions and electron rich organic molecules. The progress of the reaction is observed spectrophotometrically. The effects of different parameters like pH, change of concentration of sodium nitroprusside, concentration of ligands, light intensity etc. on percentage error was investigated. The efforts were made to minimise the percentage error and some optimum conditions were obtained. Such reaction can be used for the determination of Cystiene in the range of millimoles to micromoles, hence it is important to know whether such estimations can be done successfully and that to with the desired accuracy.