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Paper Details

Simultaneous Spectrophotometric Methods for Estimation of Levocetirizine and Ambroxol in Pharmaceutical Dosage Form

Jyostna S.Patil*, Chandan R. Rane, Radhika D.Ingale

Journal Title:Journal of Chemical, Biological and physical sciences

Three methods for simultaneous estimation of Levocetirizine dihydrochloride (LEVC) and Ambroxol hydrochloride (AMB) in combined solid dosage forms have been developed. The methods employ the application of simultaneous equation, the absorbance ratio (Q-analysis) and Area under curve methods. All these methods utilize 0.1M HCl as a solvent. LEVC shows maximum absorbance at a wavelength of 231 nm and AMB at 245 nm, where the linearity ranges for LEVC and AMB were 3-30 ?g/ ml and 5-50 ?g/ ml, respectively. Determination of ratio of absorbance at 245nm, the maximum absorption of AMB and isobestic wavelength 239 nm, the linearity ranges for LEVC and AMB were 5- 50 ?g / ml and 5- 50 ?g / ml, respectively. The third method depends on the application of area under the curve at selected wavelength range, 228-234 nm and 242-248 nm for LEVC and AMB, respectively. The linearity ranges for LEVC and AMB were 3-30 ?g/ ml and 5-50 ?g/ ml .The procedures were successfully applied for the simultaneous determination of both the drugs in laboratory prepared mixtures and in commercial capsule preparation. The accuracy of the methods was assessed by recovery studies and was found to be 99.64% 0.00522 and 99.25% 0.02006 for the simultaneous equation method, 99.38% 0.00363 and 99.28% 0.01886 for the graphical absorbance ratio method and 99.61% 0.06262 and 99.81% 0.01191 for the area under curve method, for LEVC and AMB respectively.