Synthesis and Potentiometry Determination of Stability Constant of Binary Complex of Tetrapeptide TFA.Ala- Phe-Pro-Leu-OH with D-Block Transition Metals
Sanjay N. Aher a, b,*, Supriya S. Aher b, S. D. Rathod a.
Journal Title:Journal of Chemical, Biological and physical sciences
Tetrapeptide TFA. Ala-Phe-Pro-Leu-OH synthesized by using Solid phase
peptide synthesis (SPPS). Proton-ligand formation constant (pK) of tetrapeptide have
been evaluated by potentiometry measurements using Calvin-Bjerrum titration
technique as used by Irving and Rossotti. The binary complex of Ni (II), Mn (II), and
Fe (II) with tetra peptide are examined at varying temperature and ionic strength
conditions in aqueous media. The stability of tetrapeptide metal complex with dblock
transition metals follow the Irving-Williams order and shows inverse trend with
varying temperature and ionic strength conditions.