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Paper Details

A Study on Biochemical and Molecular Methods for Diagnosis of Pulmonary Tuberculosis

Pratibha Rani Dwivedi1, Stuti shriwastava1, Seema Tiwari1*, Rashmi Arnold2, Arti Saxena3 and Umesh Pandey3

Journal Title:Journal of Chemical, Biological and physical sciences

TB is most common opportunistic disease that affects people infected with HIV. As HIV debilitates the immune system, vulnerability to TB is increased. Recently, new multi drug-resist strains of tuberculosis (MDR-TB) have developed and are spreading. A multidrug resistance strain is defined as M. tuberculosis resistant to ionized and rifampin with or without resistant to other drugs. This has resulted is many case of magically treatable, and often fatal diseases. Inadequate therapy is the most common means by which resistant bacteria are acquired and patients who have previously undergone therapy should be presumed to harbor MDR-TB, until proved otherwise. The objective of this study was to estimate the level of certain pathologically important isozyme (eg LDH) in cases (TB patients) and controls and to standardize the PCR based identification method for mycobacterial strains in DNA samples extracted from sputum specimen.