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Study of Pathogenic Bacterial Isolates from Patients with Skin Infections and their susceptibility to Antibiotics in Some Hospitals in Taiz City-Yemen

Al-Jendy, A.S.1 *,Al-Ofairi, B.A.2 *,Al-Ganady, M.H.3 1Department of Medical laboratory technology - Al-Ma’afer CommunityCollege –TaizYemen. 2Department of Biology- Microbiology, Faculty of Science, Sana’a University-Yemen. 3Department of Clinical laboratory analysis, Institute of health - Taiz –Yemen. *Corresponding Author: Ahmed Abdulla Salem Al-Jendy, Department of Medical Laboratory Technology, Al-Ma’afer Community College , Taiz-Yemen.

Journal Title:Aalborg Academy Journal of Medical Sciences

Background: Skin and soft tissue infections (SSTIs) are commonly encountered in clinical practice, the spectrum of the causative bacterial agent changes constantly and so does their antibiotics. Aim: This study was carried out to investigate the incidence of different aerobic bacteria isolates. Materials and methods: Samples were collected from patients with skin infections at a local Hospital in Taiz City, Yemen. A cross-sectional study was conducted during the period between January to December 2014. A pus specimens were collected aseptically from 130 cases of skin and soft tissue infections in the sterile condition and cultured on blood agar, MacConkey agar and mannitol-salt agar media. After growing and staining of bacteria by gram stain, bacteria were cultured in differentiated media, strains that were isolated, undergo antibiotic susceptibility test by Kirby-Bauer disc diffusion method. Results: From a total of 130 swabs collected, 89 (68. 5%) were with bacterial growth : gram positive bacteria form [57 (64%)], while gram negative bacteria were [32 (36%)], common aerobic bacterial pathogens were: S. aureus[30 (33.7%)], S. epidermidis [18 (20.2%)], P. aeruginosa [14 (15.9%)], S. pyogenes [9 (10.1%)], E. coli [8 (8.9%)], K. Pneumoniae [6 (6.7%)], P. mirabilis [4 (4.5%)]. These results showed that in vitro antibiotic susceptibility tests among gram positive cocci susceptibility was highest to Vancomycin, Fusidic acid (except S. pyogenes) and Ciprofloxacin. Among gram negative bacilli Gentamycin, Chloramphenicol and Ciprofloxacin susceptibility was high, however, all Escherichia coli isolates were resistant to Penicillin, Ampicillin, and Tetracycline. Additionally, all Proteus mirabilis isolates were resistant to Erythromycin and Penicillin. Conclusion: This study indicated that the multi drug resistance (MDR) of bacterial organisms were alarming for increase in skin infections . Keyword: Pathogenic bacteria, Skin and soft tissue infections, Antibiotic resistance, Taiz City, Yemen.