Extraction and Preliminary Characterization of Potential Antibacterial Agent from Potential Streptomyces cinereoruber sp. Isolated from an Chilika Lake
Anjani K Upadhyay, Debasmita Chatterjee, Madhuri Swain1 and Lopamudra Ray
Journal Title:Acta Scientific Microbiology
Streptomyces, isolated from marine and estuarine habitat have been widely recognized as a potential source of antifungal, anti-tumour, anti-bacterial compounds. In the present study, the antimicrobial agent production potential of a Streptomyces cinereorubersp was evaluated. The selective isolation of the strain was carried out on starch casein agar. The primary screening of the Streptomyces isolate was done by cross streak method against pathogenic test strains Escherichia coli MTCC 82, Staphylococcus aureus MTCC 96, Bacillus cereus IP406 and Salmonella typhi MTCC 734 and Micrococcus luteus and the antimicrobial property against Micrococcus luteus was confirmed. The secondary screening was carried out by using the culture supernatant against the test strain by agar well diffusion method. The growth and antimicrobial production ability of the strain against Micrococcus luteus was studied. The antimicrobial agent production was also observed till pH 11 and NaCl concentration 3% (w/v). The partially purified compound showed a peak similar to streptomycin in HPLC. The culture condition for the production of the compound was opimised.