Determination of Optimum Survivability Factors of Highly Pathogenic Vibrio cholerae 01 Serogroup-Specific Bacteriophage JSF4ϕ
Mohammod Johirul Islam1*, Titash Chandra Sarker1, Ruksana Akter Jebin1, Farjana Yasmin1, Mousumi Khatun1, Md. Ashraful Islam1, Sadia Binte Ramzan1, Fahim Alam Nobel1, Sharmin Akter1, Monira Islam1, Mohammad Mehedi Hasan1, Mst. Mahmuda Khatun1, Shahnaz Yesmin1, Saima Sabrina1, Marina Khatun1, Abdullah Al Mamun1, Md. Khairul Islam1, Mohammad Asaduzzaman2, Keshob Chandra Das3, Mohammed Badrul Amin4, and Mohammad Khaja Mafij Uddin5
1Dept. of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Santosh, Tangail, Bangladesh; 2Dept. of Biochemistry, Primeasia University, Banani, Dhaka, Bangladesh; 3Dept. of Molecular Biotechnology, National Institute of Biotechnology, Savar, Bangladesh; 4Food Microbiology Laboratory, Laboratory Sciences, and Services Division, ICDDR,B, Mohakhali, Dhaka, Bangladesh; and 5Mycobacteriology Laboratory, Infectious Disease Division, ICDDR,B, Mohakhali, Dhaka, Bangladesh
Journal Title:American Journal of Pure and Applied Biosciences
Cholera is severe watery diarrhea caused by pathogenic V. cholerae 01 or 0139 serogroups. In each year, 2.9 million people are affected by cholera worldwide and 95000 deaths occur from the disease annually. In Bangladesh, around 100000 people are affected by this disease and approximately 4500 deaths occur each year. In this study, a novel V. cholerae 01 serogroup-specific bacteriophage JSF4ϕ was used. This phage was able to lyse both the clinical and environmental pathogenic V. cholerae 01 serogroup strains and one of our previous studies demonstrated that the seasonal outbreaks of cholera caused by V. cholerae 01 serogroup strains in Bangladesh are mostly regulated by this bacteriophage. In this current study, we determined the optimum survivability factors of JSF4ϕ bacteriophages. This study showed that the temperature 2500C, pH 7, and normal saline are the optimal survivability factors for JSF4ϕ bacteriophages because, at these conditions, we have got the maximum number of plaque-forming units (PFU/mL) of these bacteriophages. This study also showed that the JSF4ϕ bacteriophages can survive at a wide range of temperature, pH, and salinity. So, the study presented here may have an impact on the controlling of cholera epidemics caused by environmental and clinical pathogenic V. cholerae 01 serogroup strains if we can use JSF4ϕ bacteriophages as a biocontrol agent. This study may also have profound implications for future studies of JSF4ϕ bacteriophages as a good food additive or in phage therapy for its efficient lysing capacity against the pathogenic V. cholerae 01 serogroup strains.