Effect of Antibiotic Susceptibility and Inhibitory Activity for the Control of Growth and Survival of Microorganisms of Extracts of Calendula officinalis
Md. Zulfiqer Shahen1, Shahriar Mahmud1*, Shaharuq Nahid Sohana1, Mehadi Hasan Rony1, Md. Abu Sayeed Imran1, Md. Abdullah Al Maruf2, Md. Afzal Ahmed Azim2, Md Monirul Islam3, Md. Rokibul Islam1, Md. Ekhlas Uddin1, and Mohammad Shah Alam4
1Dept. of Biotechnology and Genetic Engineering, Islamic University, Bangladesh; 2Dept. of Biochemistry and Molecular Biology, Jahangirnagar University, Bangladesh; 3BioIcon Group, Uttara, Dhaka, Bangladesh; 4Dept. of Microbiology, Gono Bishwabidyalay, Dhaka, Bangladesh
Journal Title:European Journal of Medical and Health Sciences
Extracts from many types of plants show several evidences of beneficial health effects in the living system. Calendula officinalis is a well known medicinal plant in our country. The purpose of this study was to examine the effectiveness of C. officinalis for control of the growth and survival of microorganisms. The inhibition of growth was tested by the paper disc agar diffusion method. Minimum inhibitory concentration (MIC) was determined by the tube dilution method. C. officinalis leaf extract showed inhibition (MIC, inhibitory) to Escherichia coli, Klebsiella pneumoniae, and Bacillus subtilis and to the other bacteria tested. Antimicrobial effects of leaf extract of C. officinalis on some microorganisms including pathogens were investigated. The extract of C. officinalis leaf which is prepared in was tested on bacterial cultures such as B. subtilis, S. lutea, E. coli, K. pneumoniae and also investigated that minimum inhibitory concentration 4µg/ml of leaf extract of C. officinalis in petroleum ether against K. pneumoniae and largest inhibitory zone are created by 512mg/ml chloroform extract against E. coli leaf extract of C. officinalis in petroleum ether has proved better for antibacterial activity. This study demonstrates that the potentiality of C. officinalis as a source of antimicrobials that could be harness for use in the health care delivery process.