Antibiotic Resistance Analysis of Vibrio spp Isolated from Different Types of Water Sources of Bangladesh and Their Characterization
Milon Kumar Das Sarker1, Tanvir Ahammed2, Md. Sahabuddin3, Pinki Akter1, Azizul Haque4, Md. Rajib Hossain5, Md. Golam Mosaib6, Md. Robiul Islam7, Goutom Chandra Mondol8*, and Md. Firoz Alam9
1Dept. of Microbiology, Stamford University Bangladesh, Bangladesh; 2Dept. of Microbiology and Hygiene, Bangladesh Agricultural University, Bangladesh; 3Dept. of Biotechnology and Genetic Engineering, BSMRSTU, Bangladesh; 4Dept. of Microbiology, University of Chittagong, Bangladesh; 5Dept. of Environmental Science & Disaster Management, BSMRSTU, Bangladesh; 6Dept. of Biochemistry and Molecular Biology, Gono Bishwabidyalay, Bangladesh; 7Dept. of Microbiology, Jagannath University, Bangladesh; 8Department of Public Health, ASA University, Bangladesh, and 9Dept. of Biotechnology and Genetic Engineering, Islamic University, Bangladesh
Journal Title:European Journal of Medical and Health Sciences
Cholera is an acute form of the diarrheal disease that plagued human civilization over the centuries. The enormity of human sufferings led clinicians and scientists to carry out extensive research on cholera and Vibrio cholerae leading to major discoveries that opened up novel areas of research or new disciplines in biomedical sciences. An attempt is made here to summarize some of these breakthroughs and outline their significance in broader perspectives. In the present study, a total of 12 samples were collected from four types of water sources for the isolation of Vibrio spp. Water samples are enrichment into alkaline peptone water then inoculate into culture media such as Nutrient agar, MacConkey, and Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar medium. After incubation of TCBS plates for 24h at 37°C yellow and green colonies are screened out for biochemical identification. No-sucrose fermenting Vibrio parahaemolyticus, Vibrio mimicus, and Vibrio vulnificus show green colonies and sucrose fermenting Vibrio cholerae and Vibrio alginolyticus show yellow colonies on TCBS. To identify the Vibrio spp isolates biochemical test was carried out and typical Vibrio spp give a positive result. The majority of Vibrio spp are avirulent, but certain strains may be sporadically human pathogenic. The antibiotic resistance studies showed that among the 9 isolates were resistant against Erythromycin, Penicillin, Cephalexin, Vancomycin, and 4 isolates resistant against Tetracycline. To expand current knowledge of the occurrence, ecological niche, and persistence of potential human pathogenic Vibrio spp in aquatic environments, occurrence, and laboratory studies were performed.