A Simulation Study on the Effects of Media Composition on the Growth Rate of Escherichia coli MG1655 Using iAF1260 Model
Kar Chi Cheong, Raphael YH Hon, Clara J Sander, Irwin ZL Ang, Jun Hang Foong, and Maurice HT Ling.
Journal Title:Acta Scientific Microbiology
Media compositions are important determinants of growth rate and genome-scale models (GSMs) had been used for optimizing media for metabolite production and growth. Recently, iAF1260, a GSM based on Escherichia coli MG1655, was used to study the effects varying glucose concentration in media on growth rate and metabolic fluxes. In this study, the effects of other media components in the presence of varying glucose concentrations on the predicted growth rate of E. coli MG1655 were examined. Our results show that 10 media components (ammonium, calcium, chloride, copper, glucose, manganese, magnesium, molybdate, phosphate, and potassium) demonstrate substantial impact on the predicted growth rate of E. coli MG1655. Of which, 4 components (glucose, ammonium, magnesium, and phosphate) have the most impact. However, our results also demonstrate the limitations of iAF1260 as media components that had been shown to affect E. coli growth rate were not reflected by the model.