DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR DETERMINATION OF OLANZAPINE IN PHARMACEUTICAL DOSAGE FORMS
Sunil Yadav, prakash pant, Vaibhav Verma
Journal Title:International Journal of Medical Studies
Background: Spectrophotometric analysis fulfils requirement where the simultaneous estimation of the drug
combination can be done with similar effectiveness as that of chromatographic methods. Simultaneous
estimation of drug combination is generally done by separation using chromatographic methods like HPLC, GC
and HPTLC etc. These methods are accurate and precise with good reproducibility, but the cost of analysis is
quite high owing to expensive instrumentation, reagent and expertise. Hence it is advisable to develop simpler
and cost-effective method for simultaneous estimation of drugs for routine analysis of formulation.
Methods: A descriptive study design was used and information was obtained through various literature reviews.
RP-HPLC method was used and data was analyzed.
Conclusion: The developed stability indicating HPLC method for quantitative estimation of olanzapine in bulk
and pharmaceutical dosage forms is fast, simple, accurate, and more precise. Validation of this method was
accomplished, getting results meeting all requirements. Thus, the developed HPLC method can be used for
routine quality control test.
Keywords: Chromatography, RP-HPLC, Spectrophotometric, Simultaneous estimation.