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Paper Details

Non - Invasive Monitoring and Assessment of Phage Therapy and Phage Targeting in Bovine Brucellosis Employing a Brucella - Specific Biomarker

Anju Mohan and Hari Mohan Saxena

Journal Title:Acta Scientific Microbiology

Brucellosis caused by Brucella organisms is a major zoonosis globally. It causes heavy losses through abortions, delayed concep-tion and infertility in animals. Antibiotic therapy is ineffective. Once infected, the animal remains carrier and sheds bacteria in milk, semen and uterine discharges for long period. We have successfully used a lytic brucella phage for therapy of Brucellosis in adult cattle. We also targeted the phage employing the live attenuated Brucella abortus strain 19 organisms to kill the virulent Brucellaresiding intracellularly in phagocytes in the body. The effect of both the therapies was monitored non-invasively employing BrucellaRNA in blood plasma as a Brucella - specific biomarker. A single dose of the phage alone or the therapeutic vaccine (phage pulsed S-19) could substantially reduce and finally eliminate live Brucella in the body within 3 months as evident from diminished and ulti-mately non – detectable RNA characteristic of Brucella abortus (223 bp amplicon) in plasma by RT-PCR. Thus, phage has a potential to cure Brucellosis and abolish carrier state in cattle and RNA can serve as a specific biomarker of live Brucella for monitoring and assessment of the efficacy of the therapy