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Paper Details

Strain Improvement of Saccharomyces cerevisiae by Bridge-induced Chromosome Translocation (BIT) and YAC Recombineering Technology

Burcin Altun, Valentina Tosato and Carlo V Bruschi

Journal Title:Acta Scientific Microbiology

The present research deals with implementation of the bridge-induced chromosome translocation (BIT) technology and yeast artificial chromosome (YAC) recombineering for strain improvement of bioethanol producing transgenic yeast. We aimed to construct a YAC that carries cellulose degradation genes on it and then to apply the BIT technology. BIT technology allowed us to gain two advantages; one of them was to stabilize YAC into the yeast genome and the other one was to have increased a gene expression level consequent to the translocation event. Selection strategies were implemented to obtain novel genetic regulation that would achieved the final phenotype originally desired with the high cellulose degradation and high ethanol producing features.In conclusion, in our study, we utilized two novel technologies (Yeast Artificial Chromosome (YAC) recombineering and Bridge-Induced Translocation (BIT) technology to introduce new, multi-factorial genetic traits into a yeast strain, a process that would otherwise take several time-consuming and labor-intensive rounds of genetic engineering. This work describes the successful recombinant translocant yeast that is able to efficiently utilize cellulosic material as a carbon source with highly stable recombinant translocant chromosome and has high level of cellulases capacity