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Paper Details

Spectrum and Antibiogram of Bacteria Isolated from Commercially Available Stockfish in Eke-Awka Market, Anambra Nigeria

Malachy C Ugwu, Ebube Ofoegbu and Kene C Ezejiegu

Journal Title:Acta Scientific Microbiology

Background: Food borne infections and diseases remain a major cause of morbidity and mortality, particularly among poor/developing countries. Food products play significant role in the transfer of antibiotic resistance. There is increasing evidence of the use of antibiotics in fish rearing as growth promoters or curative agents and this could promote antibiotic selective pressure which results in emergence of resistant organisms in these sea foods. This study is aimed at isolating and determining the spectrum and antibiogram of bacteria isolated from commercially available stock fishes in eke Awka market. Method: The isolated samples were obtained from randomly selected samples of stock fishes using pour plate method and confirmed with the help of microscopy and biochemical test. The antibiogram of the isolates were examined following Clinical and Laboratory Standard Institute breakpoints. Results: A total of 70 samples of stock fishes were collected from the market. Staphylococcus aureus was the most contaminant (45.7%) in the samples, followed by Klebsiella pneumoniae (41.1%), Bacillus subtilis (30%), Escherichia coli (15.7%), Salmonella typhi (5.7%) and Pseudomonas aeruginosa (4.3%). The isolates showed resistance to most of antibiotics used. Gentamicin and Ofloxacin had appreciable activity against all Gram positive isolated bacteria while Gentamicin and Nitrofurantoin having activity against some Gram negative bacteria. Conclusion: The study confirmed the presence of antibiotics-resistant bacteria in stock fishes, making it a potential public health threat. This calls for improved hygienic practices among the fish vendors and there should be rational use of antibiotics in fish farming